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    BBU Resources

         SGI Workstations:


    BLL Cell Imaging Unit (BLL-CIU)

         Confocal Microscopes:
    • Leica TCS SP2 AOBS
            Laser scanning confocal upright microscope with a temperature-controlled environmental chamber for live-cell
            imaging and bleaching (FRAP) applications. Available laser lines are 458, 476, 488, 496, 514, 561 and 633 nm,
            spectral detectors for five channels
      ...   More Details

         Live cell imaging:
    • TILL Photonics wide-field
            Fast inverted live-cell imaging system with multi-wavelength monochromator excitation range of 320-680 nm.
            Equipped with a temperature-controlled environmental chamber for prolonged image acquisition protocols
      ...   More Details

         PC Workstations:
    • Analysis workstation 1
            Dell Precision 650 with dual Xeon 32-bit 3.06 GHz CPU, 3 GB RAM, dual monitors, Leica LCS software, Adobe
            Design Premium CS3 suite, and various image analysis, statistical and graphing software
      ...   More Details

    • Analysis workstation 2
            HP xw9400 with quadruple AMD Opteron 64-bit 3.0 GHz CPU, 16 GB RAM, dual monitors, Leica LCS software, SVI
            Huygens Pro deconvolution, Bitplane Imaris 3D rendering, and other image analysis, statistical and graphing
            software
      ...   More Details

         TIRF microscope:


    FACS CORE FACILITY

         Flow cytometers:
    • FACSAria
            BD FACSAria has three lasers: blue 488 nm, red 633 nm and violet 407 nm. You can analyze up to 11 fluorescence
            and 2 scatter parameters simultaneously. Cells can be sorted into 1 ml tubes, 12 x 75 mm tubes, 15 ml falcon
            tubes, into various plates.


    • FACSArray
            96-well plate Flow cytometer, has yellow (532nm) and red (635nm) lasers and 4 detection channels


    High Throughput Center

         Colony Picker:
    • K6 Bioctopick Colony Picker
            KBiosystems K6 bacteria colony picker for HT cloning purposes. Can separate betwee blue and white.

         Flow cytometers:
    • FACSArray (GFP)
            96 well plate Flow cytometer, has green(480nm) and red (635nm) lasers and 4 detection channels....   More Details


         Instructor:
    • James Thompson
            Can assist in design of assay and automation.

         Laser Scanning Cytometer:
    • Acumen eX3
            The Acumen eX3 is a laser-scanning fluorescence microplate cytometer comprising a 488 nm solid state laser.
            The instrument has two channels at 500-530 to detect and a second channel from 522-600 to detect yellows and
            reds (see HTC Tables). The non-confoca


         Liquid handling:
    • Biomek FX
            High Throughput liquid handling instrument having 96-dispensing head and 96 and 384 pintool for chemical or
            bacteria transferring.
      ...   More Details



    • Thermo CRS/Multidrop
            Thermo CRS liquid handling robot for simple applications. Feasible for cell spreading to microwell plates (96
            or 384).
      ...   More Details

         Mammalian cell culture facility:
    • Mammalian cell culture facility
            Cell culture facility for high throughput experiments. Includes laminar, +37 C, 5% CO2 incubators, and +22 C,
            +4 C and -20 C reagent storage capacities


         Multilabel readers:
    • Fluostar Optima 1
            Multilabel reader with extensive filterset(>20 filters: A260/A280, A600, Hoechst, GFP, etc.). Detection
            technologies include: Fluorescence, Luminescence, Flash Luminescence, Absorbance and UV-absorbance. UV-Abs can
            be used for measuring DNA concentration
      ...   More Details


    • Topcount Luminometer
            PerkinElmer Topcount for HTS luminometry. Reads 96/384 -well plates.

         PCR instrument:


         PCR set-up room/Pilot phase trails:
    • PCR set-up room
            Set-up PCR reactions in a clean environment, use latest demonstration instruments from commercial sources

         Plasmid purification station:
    • Biorobot 8000
            Suitable for plasmid purification and some other filtration assays, like His-tag purifications. FOC usage
            until 15.1.2007



    KTL Cell Imaging Unit (KTL-CIU)

         Microscopes:


    MCB/GSB Research Programs

         Microscopes:




    Molecular Imaging Unit (MIU) [MOVED]

         Accessories:

    • Temperature controller
            Temperature control unit for Stallion objective heater and Meta confocal stage heater

         Confocal Microscopes:


         High Content Screening Systems:

         Live cell imaging:

         Microscopes:

         PC Workstations:



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